Nanoscale Metal–Organic Frameworks for Real-Time Intracellular pH Sensing in Live Cells
نویسندگان
چکیده
Real-time measurement of intracellular pH in live cells is of great importance for understanding physiological/pathological processes and developing intracellular drug delivery systems. We report here the first use of nanoscale metal-organic frameworks (NMOFs) for intracellular pH sensing in live cells. Fluorescein isothiocyanate (FITC) was covalently conjugated to a UiO NMOF to afford F-UiO NMOFs with exceptionally high FITC loadings, efficient fluorescence, and excellent ratiometric pH-sensing properties. Upon rapid and efficient endocytosis, F-UiO remained structurally intact inside endosomes. Live cell imaging studies revealed endo- and exocytosis of F-UiO and endosome acidification in real time. Fluorescently labeled NMOFs thus represent a new class of nanosensors for intracellular pH sensing and provide an excellent tool for studying NMOF-cell interactions.
منابع مشابه
Synthesis and Characterization of Nano-Structure Copper Oxide From Two Different Copper (II) Metal-Organic Framework Precursors
Nano-structured copper oxides were successfully prepared through direct calcination of 1D ladderlike metal-organic framework [Cu2(btec)(2,2'-bipy)2]∞, (btec = 1,2,4,5-benzenetetracarboxylate and 2,2'-bipy = 2,2'-bipyridine) and porous coordination polymer [Cu(BDC)(bipy)](BDCH2), (BDC = 1,4-benzenedicarboxylate; bipy = 4,4'-bipyridine). The nano-structure of the as-synthesized samples are charac...
متن کاملAn amine/imine functionalized microporous MOF as a new fluorescent probe exhibiting selective sensing of Fe3+ and Al3+ over mixed metal ions
Nowadays metal-organic frameworks with multiple luminescent centers are very fascinating as multifunctional luminescent material because of their luminescence properties, which could be systematically tuned by deliberate use of organic ligands and metal ions. In this research, we explored a microporous mixed-ligand MOF for highly selective and sensitive detection of metal ions. A two-fold inter...
متن کاملSynthesis of Different Copper Oxide Nano-Structures From Direct Thermal Decomposition of Porous Copper(ΙΙ) Metal-Organic Framework Precursors
Copper oxide nanostructures have been successfully synthesized via one-step solid-state thermolysis of two metal-organic frameworks, [Cu3(btc)2] (1) and [Cu(tpa).(dmf)] (2), (btc = benzene-1,3,5-tricarboxylate, tpa = therephtalic acid = 1,4-benzendicarboxylic acid and dmf = dimethyl formamide) under air atmosphere at 400, 500, and 600°C. It has also been found that the reaction temperature pla...
متن کاملInfluence of amine group on the adsorptive removal of basic dyes from water using two nanoporous isoreticular Zn(II)-based metal organic frameworks
Dyes are the most abundant hazardous components existing in the environment because of their extensive use in industries. So, in the present study, two isoreticular Zn(II)-MOFs, TMU-16 and TMU-16-NH2, were used for the adsorptive removal of harmful cationic dyes from aquatic medium. In order ...
متن کاملFar-field photostable optical nanoscopy (PHOTON) for real-time super-resolution single-molecular imaging of signaling pathways of single live cells.
Cellular signaling pathways play crucial roles in cellular functions and design of effective therapies. Unfortunately, study of cellular signaling pathways remains formidably challenging because sophisticated cascades are involved, and a few molecules are sufficient to trigger signaling responses of a single cell. Here we report the development of far-field photostable-optical-nanoscopy (PHOTON...
متن کامل